Fluorescent biomarker dyes have a very broad application prospect in the field of biomedicine. Especially, fluorescence recognition and fluorescence imaging are fast, safe, efficient and non-invasive. They are very suitable for early diagnosis and guiding treatment of diseases in tissues or in vivo.
Recently, Zhu Weihong's research group of Chemistry College of East China University of Science and Technology has made important progress in the research of near-infrared fluorescent recognition dyes. It has successfully realized real-time in vivo and in situ detection of β-galactosidase and obtained high-resolution three-dimensional in vivo imaging. The signal is used to accurately locate the tumor tissue. The related research results are published online in "American Society of Chemistry" by "Real-Time Tracking and In Vivo Visualization of β-Galactosidase Activity in Colorectal Tumor with a Ratiometric Near-Infrared Fluorescent Probe". The work was mainly completed by doctoral students Gu Kaizhi and Associate Professor Guo Zhiqian, and was greatly assisted by Professor Shi Ping from the School of Bioengineering.
Β-galactosidase is an important marker of cell aging process and related diseases such as primary ovarian cancer. The benzopyranonitrile fluorophore is a near-infrared dye signal group, and the group skillfully constructed a pair of β- A near-infrared ratio fluorescent probe specifically recognized by galactosidase, which exhibits both ratio-type and enhanced near-infrared signal response when detecting β-galactosidase, successfully achieving endogenous β in living cells. - Quantitative detection of galactosidase, using near-infrared specific, highly sensitive fluorescence response signal to achieve accurate localization of tumor tissue overexpressing β-galactosidase.
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