1. Q: What is the significance of measuring the cell growth curve?
A: The cell growth curve is a common method for determining the absolute growth of cells. It is also an important indicator for determining cell viability and one of the basic parameters for culturing cell biological characteristics. Since the cells are too small to measure the growth state of individual cells, the growth curve of the cell population is measured.
2. Q: How to choose the number of cell inoculation?
A: It can be calculated according to the number of inoculations and cell growth cycle during cell subculture. The number of cell inoculation varies with cells.
3. Why should I try to stain with trypan blue when counting cells?
A: After adding trypan blue, the living cells are not colored, and the cells colored by trypan blue are dark blue, which are unhealthy or dead cells and cannot be counted.
4. Why do you need to repeat holes when measuring cell growth curve?
A: When measuring the cell growth curve cell count, select 3 duplicate wells, each of which is counted three times, and the average value is taken to reduce the operation error.
5. What is the measurement period of the cell growth curve?
A: It is usually one week.
6. Why did the cell growth curve drawn did not reach the plateau?
A: There may be the following reasons: First, the cell seeding density is too low; second, the cell culture time is too short; third, the cell growth state is not good, so that it cannot proliferate normally.
7. Why does the number of cells decrease on the first day of cell counting, but decrease?
A: After the cells are passaged, there will be a slow-growing incubation period. The cells will not proliferate, and the cells will die normally. Therefore, the number of cells does not increase but decreases.
8. Is there any other way to draw the cell growth curve?
A: Of course. We mention the most direct counting method, in addition to the relative counting method, this method first draws a standard curve, calibrates the cell number and a variable function relationship, then we only need to measure the daily variable The value can be used to calculate the number of cells. Commonly used are MTT, CCK8 and other colorimetric methods.
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