High temperature weather for white shrimp farmers in South America

In high temperature weather, Penaeus vannamei, when the water temperature of the shed is higher than 30°C, the dissolved oxygen in the water is relatively stable, but the dissolved oxygen in the larvae bags is higher. If the shrimp is directly put into the breeding shed at this time , It will lead to instant emergency response of shrimp, usually called bubble disease, which will greatly reduce the survival rate of shrimp seedlings.

The correct way to shoot seedlings is to use a 60 cm round pot, put the water in the shed in a pot, dissolve it in water with some anti-emergency VC and glucose, and release the shrimp for a while (2 minutes to 3 minutes). The high-pressure oxygen in the packaging bag is volatilized, and then the shrimp is poured into the pot, so that the shrimp can fully adapt to the water temperature and then cast into the shed. At the same time, the entire pool is splashed with anti-emergency VC to ensure that the vaccine is absolutely safe!

Tips: Before the seedlings must be tested in the water of the indicators, especially the residual chlorine, do not cast seedlings before fully eliminated.

DNA/RNA Purification Kit

The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination.

Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.

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