Every year from mid-February to late April is the breeding season of cockroaches. The female quail only has estrus once in the estrus season, and mating should be carried out during the estrus. But what if the mother does not have heat? For those who are late or not in heat, artificial aphrodisiac can be performed. The method is as follows:
1. Male and female cockroaches are kept adjacent. Because the male deer is a sexual stimuli to the mother-in-law, it can lead to the growth and maturation of the follicles on the ovary of the mother-in-law. It can express estrus and receive breeding.
2. Use gonadotropin to aphrodisiac, that is, to infuse 30 to 50 grams of FSH (Follicle Stimulating Hormone) intramuscularly twice a day (in the morning and afternoon) for 2 days without estrus. In the case of estrus, one injection of LH (Luteinizing hormone) ) 25 ml to 30 ml or HCG (human chorionic gonadotropin) 100 ml to 150 ml. Or intramuscular injection of PMSG (pregnant horse serum gonadotropin) 200 ml, 2 consecutive times. Each mother-injected muscle can also be given 2 ml of triple hormone. Effectiveness can reach 90% to 95% or more.
3. Chinese and Western medicine aphrodisiac: (1) Each female quail oral vitamin E pills 1 to 2 tablets a day, once every 3 days to 5 days, with aphrodisiac effect. (2) Internally taken Chinese herb Epimedium 15g~20g, fed twice a day in the morning and evening, once every 3 days, the effect is remarkable. (3) internal medicine: Motherwort 10 grams, 8 grams of pumpkin leaves, 5 grams of safflower, Jianshui irrigation service, or feed mixed feed. For every 2 days, one dose is applied, and 2 to 3 pay. (4) Take Xiang Fu, Angelica each 10 grams, Angelica 12 grams, Chuanxiong 8 grams, decoction after a gavage, the effect is significant.
4. Increase lighting. In areas where there is a short day of sunshine, or in a dark place where cages are kept, provide sufficient light to make the daily light time reach 14 hours to 16 hours, which has a good aphrodisiac effect.
5. Malt aphrodisiac. Two to three days after germination, the wheat buds were mixed into the concentrate and fed to the female quail, two times a day, 200 g to 250 g each for 3 days. Germ is rich in vitamin C and vitamin B and vitamin E, as well as some active substances, with good aphrodisiac effect. Feeding carrots also has a good aphrodisiac effect (because carrots are rich in carotene can be converted into vitamin A in the quail, can promote follicular development).
6. Steam out a steamed bun 1, vinegar and a half bowl. Divide the steamed buns into two halves, simmer in the vinegar, and let the clams eat after being cold. Use 3 times in a row.
7. Take 150g~200g of brown sugar, put it in the pot, add water decoction, and mix it into the feed. Generally 2 days to 7 days after feeding, the mother will have heat and receive breeding.
8. Oral administration of 20 grams of pine needle bioactive substance (also known as pine needle chlorophyll, carotene) daily for 5 days to 7 days, can make the ovary active, express estrus, and can be bred.
In addition to the aforesaid methods applied to non-estrus mothers, some of the above methods can be implemented by referring to foxes, dogs, cockroaches, cows, sheep, rabbits, etc. (The dosage can be increased or decreased according to body size. ).
Extraction Kit Magnetics Bead Method
This standard specifies the terms and definitions, classification, technical requirements, test methods, identification, labels and instructions for use, packaging, transportation and storage of nucleic acid extraction kits (magnetic beads method) (hereinafter referred to as "kits"). This standard It is suitable for extracting and purifying human genomic nucleic acid and its fragments from various clinical samples such as serum, plasma, whole blood, cerebrospinal fluid, milk, saliva, urine, sputum, swab, tissue or paraffin-embedded tissue by magnetic bead method , Pathogen nucleic acid kit. Pathogen nucleic acid includes deoxyribonucleic acid (DNA), ribonucleic acid (RNA), etc. This standard does not apply to nucleic acid extraction reagents contained in closed systems that cannot extract nucleic acid extraction products for detection.
This kit uses magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality virus from serum, plasma, lymph, cell-free body fluid, cell culture supernatant, urine or various virus preservation solutions DNA/RNA. The uniquely embedded magnetic beads have a strong affinity for nucleic acids under certain conditions, and when the conditions change, the magnetic beads release the adsorbed nucleic acids, which can achieve the purpose of rapid separation and purification of nucleic acids. The whole process is safe and convenient, and the extracted viral DNA/RNA has high yield, high purity, stable and reliable quality, and is especially suitable for automated extraction of high-throughput workstations. The nucleic acid purified by this kit can be applied to various routine operations, including RT-PCR, fluorescence quantitative PCR and other downstream experiments.
Nucleic Acid Lsolation Reagent Kits,Dna Purification Kit,Viral Rna Extraction Kits,Nucleic Acid Extraction Reagents
Jilin Sinoscience Technology Co. LTD , https://www.jilinsinoscience.com