Introduction
Sudan red dye is a kind of diazo coupling compound, insoluble in water, and can be used as a coloring agent for waxes, oils, solvents and plastics. Most of the dyes, either alone or in combination, form a color very similar to the natural colored compounds in red peppers (see Figure 1 for the structure of the two important capsicum pigments). However, the potential carcinogen of Sudan red dye is sometimes used to color the pepper products. Since such use is illegal, no Sudan red dye is allowed in the food. Therefore, a sensitive and reproducible method has been developed to determine Sudan red dyes in typical pepper products. However, the base of capsicum oil is more complex than fresh pepper or paprika. Chili oil is a high concentration mixture of natural oils and resins extracted from peppers. It is used as a strong flavoring agent and a strong colorant in the food industry. It is also used as a source of capsaicin in pharmaceutical preparations and pepper sprays. Since chili oil can be used as a food colorant, an efficient analytical method is needed to determine illegal dyes in foods. This application note presents an SPE analysis method suitable for the determination of Sudan red dyes commonly found in capsicum oil. This application note also describes a liquid phase method using a solid core analytical column packed with 2.7 μm particles. This column provides efficient separation at lower back pressures and is compatible with HPLC and UPLC ® systems. Figure 2 shows the structure of the dye used in this study.
Sample Preparation
Chili oil (chili) was purchased from commercial sources. 0.1 g of this resin sample was dissolved in 1 mL of hexane. A Sep-Pak silica gel cartridge (6 mL, 500 mg, part number WAT043400) was activated with 3 mL of hexane and the sample was diluted. The cartridge was washed with 2 mL of hexane and then eluted with 2 mL acetonitrile/dichloromethane (5:95). The eluate was evaporated to dryness and reconstituted with 150 μL of methanol. The SPE uses a vacuum tube with the lowest vacuum and the liquid can pass through the column at a flow rate of 2 mL/min in all steps.
Results and discussion
The results are shown in Table 2. The Sudan red dyes determined in this study include phenols (such as Sudan Red No. 1), polyphenols (such as Sudan Orange G), and amines (such as Sudan Black). The Sep-Pak silica gel cartridge was selected for SPE purification because it effectively retains 10 target analyte dyes. Although the recovery is generally between 75% and 100%, the recovery of polyphenols Sudan Orange G is only about 50%. This compound is more retentive on silica gel SPE cartridges than other dyes. The recovery can be increased by increasing the elution volume or increasing the percentage of acetonitrile in the elution solvent. However, these steps result in an increase in the content of the dark matrix compound in the final extract, so that the purification effect is remarkably deteriorated. Figure 3 shows the obtained purified sample. The left sample vial is a 150 μL sample taken from a 1.0 mL sample dissolved in hexane before SPE treatment. The right sample vial is reconstituted in 150 μL after SPE treatment. A sample of methanol. The SPE solution not only significantly improved the purity of the extract, but also concentrated the extract nearly 7 times.
The CORT ECS 2.7 μm solid particle column provides excellent chromatographic performance and consistent performance over 200 injections. Figure 4 shows a typical analytical chromatogram of a spiked 100 ng/g (ppb) sample. This analysis was performed on an ACQUITY UPLC H-Class system. This system is designed to provide excellent performance not only at UPLC pressures and flow rates, but also under HPLC conditions such as those used in this study. Since the column back pressure observed in this study was approximately 3100 psi, analysis on conventional HPLC instruments with a pressure upper limit of 4000 psi or higher did not present a problem. For analysts who require true UPLC performance, the separation can be transferred to a CORTECS C 18 , 1.6 μm solid column for analysis.
in conclusion
â– An effective method for the determination of Sudan red dye in chili oil was developed.
â– Sep-Pak silica cartridges are available for efficient sample purification.
■CORTECS C 18 , 2.7 μm solid column provides excellent chromatographic performance and back pressure compatible with most traditional HPLC systems.
Pain Relief Patch for Breast
[Name] Medical Cold Patch
[Package Dimension] 10 round pieces
The pain relief patch is composed of three layers, namely, backing lining, middle gel and protective film. It is free from pharmacological, immunological or metabolic ingredients.
[Scope of Application] For cold physiotherapy, closed soft tissue only.
[Indications]
The patches give fast acting pain relief for breast hyperplasia, breast fibroids, mastitis, breast agglomera tion, swollen pain.
[How To Use a Patch]
Please follow the Schematic Diagram. One piece, one time.
The curing effect of each piece can last for 6-8 hours.
[Attention]
Do not apply the patch on the problematic skin, such as wounds, eczema, dermatitis,or in the eyes. People allergic to herbs and the pregnant are advised not to use the medication. If swelling or irritation occurs, please stop using and if any of these effects persist or worsen.notify your doctor or pharmacist promptly. Children using the patch must be supervised by adults.
[Storage Conditions]
Store below 30c in a dry place away from heat and direct sunlight.
Pain Relief Patch For Breast,Pain Relief Plaster For Breast,Relief For Breast Pain,Pad Relief Patch For Breast
Shandong XiJieYiTong International Trade Co.,Ltd. , https://www.xijieyitongpatches.com