Liquid chromatography mobile phases are typically stored in glass, Teflon or stainless steel containers and cannot be stored in plastic containers. Because many organic solvents such as methanol, acetic acid, etc. can leach out of the plasticizer in the plastic, the solvent is contaminated. Contaminated solvents such as those used in liquid chromatograph systems may cause reduced column efficiency. The storage container must be tightly sealed to prevent changes in composition caused by solvent volatilization, while preventing oxygen and carbon dioxide in the air from dissolving into the mobile phase.
Phosphate and acetate buffers are prone to mildew and should be prepared as freshly as possible. For storage, it can be refrigerated in the refrigerator and generally should not be more than 3 days.
The halogenated solvent may contain traces of acidic impurities that can react with the stainless steel in the liquid chromatograph system. A mixture of a halogenated solvent and water is more easily decomposed and cannot be stored for too long. After the halogenated solvent is mixed, it may react to form some compounds which are highly corrosive to stainless steel. Therefore, try not to use a mixed mobile phase with a halogenated solvent, or freshly prepared before use.
In addition, the liquid chromatograph may also crystallize when the halogenated solvent is mixed with some reactive organic solvent such as acetonitrile.
The reservoir is a container with a premixed mobile phase or mobile phase component that is relatively simple to construct, but can also be problematic for use with improper reservoirs. Contamination of the reservoir may block the sintered stainless steel filter, affecting the performance of the liquid chromatograph pump, causing unwanted peaks or noise in the chromatogram.
The volume of the reservoir is generally 1L. Some reservoirs can only be used for flushing gas, but not for vacuum degassing because of the risk of cracking. The reservoir for vacuum degassing should be thickened or jacketed. The self-contained reservoir should be loosely covered to prevent dust. Too tight a cover creates a partial vacuum that stops the pump.
Diagnostic reagents can be divided into two categories: in vivo diagnostic reagents and in vitro diagnostic reagents. It is mostly a reagent for detection by the reaction between antigen and antibody.
A: Classification of in vitro diagnostic reagents:
1. In vitro biodiagnostic reagents managed as drugs include:
1. Blood type and tissue type reagents;
2. Microbial antigen, antibody and nucleic acid detection reagents;
3. Tumor marker reagents;
4. Immunohistochemistry and human tissue cell reagents;
5. Human genetic testing reagents;
6. Biochips;
7. Allergy diagnostic reagents.
2. In vitro reagents managed as medical devices include:
1. Clinical basic test reagents;
2. Clinical chemistry reagents;
3. Blood gas and electrolyte determination reagents;
4. Vitamin determination reagents;
5. Cell histochemical stains;
6. Autoimmune diagnostic reagents;
7. Microbiological test reagents.
B: According to medical test items, clinical diagnostic reagents can be roughly divided into clinical chemical test reagents, immunology and
Serological testing reagents, hematological and cytogenetic testing reagents, microbiological testing reagents, body fluid excretion
Detection reagents, genetic diagnosis reagents, etc. Among them, the market share of clinical chemistry
The largest, close to 34%; followed by the immunology market, accounting for about 29%. Novel immunodiagnostic reagents and genetic diagnostic tests
The reagent was developed in the late 1980s, and it is the most common diagnostic reagent for all current diagnostic reagents, regardless of technology or market.
The fastest growing product.
Urine Rapid Test Kit,Rapid Test Kit 6-Panel,Toxoplasma rapid test kits,Fecal Occult Blood Test
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