Release date: 2015-03-05
Recently, Oxford Nanopore Technologies announced that it will invite more users to join the early trial of the MinIon sequencer. At the same time, more and more early adopters have released their findings.
The MinION sequencer is only 4 inches long and is the size of a normal U disk. It consists of a sensor chip, an ASIC and a complete flow control system for single-molecule sensing. MinION uses a novel nanopore sequencing with an average read length of 80K bp and high sequencing speed. At present, the low sequencing accuracy of nanopore sequencing is still a concern, but the device is still being updated, and many researchers are very optimistic about this technology.
Last week, at the Genomics Biotechnology Conference (AGBT) in Marco Island, Florida, Sara Goodwin from the Cold Spring Harbor Laboratory in the United States demonstrated de novo assembly and sequencing using the Oxford MinION handheld sequencer. The study used MinION to sequence more than 120X coverage of yeast strains. The results showed that the sequencing data could reach 80% alignment rate.
Users of other early trial programs are also in the process of promulgating trials of the MinION handheld sequencer. Recently, the Mark Akeson research team at the University of California, San José, published a single-nucleotide mutation detection tool for nanopore data that can be used to solve a cancer gene copy number problem in an unknown region of human chromosomes. .
The results of some research groups showed that the sequencing of the bacterial genome was performed using MinIon, and the short sequence detected by the Illumina sequencer was used to correct it. The data obtained were sufficient for de novo assembly of the genome. The published results also include metagenomic sequencing, human gene sequencing, bacterial antibiotic resistance identification, and bacterial outbreak analysis.
At the end of 2013, Oxford Nano-holes opened its first application for the MinIon trial program. So far, it has accepted two rounds of participants who will receive the MinIon sequencer in the spring of the next year. The early trial program has been focused on the field of DNA sequencing, but the company said, "I hope to expand the analysis of RNA and protein in the future."
Oxford Nanopore claims that the company's sequencing technology has improved in some areas while the early trial program is being implemented. Library preparation kits have been upgraded several times to improve data accuracy and streamline operations. The company has also developed new library preparation kits and library preparation methods to make the application of MinIon more extensive, such as cDNA sequencing and barcode encoding of genomic DNA and amplicons.
To date, Oxford Nano has been loaded with three types of flow cells, each increasing the number of nanopore to increase the quality and quantity of sequencing data. In terms of data quality, the accuracy of sequencing will be increased by increasing the proportion of bidirectional sequences.
Source: Sequencing China
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