Total Bilirubin (T-Bil) Assay Kit/Test Kit/Ivd Reagents

Model NO.: T-Bil
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*60 mL, R2: 2*15 mL
Origin: Jinan, Shandong
Model NO.: T-Bil
Application: Hospital, Lab, School
Trademark: Biomedia
Specification: R1: 2*60 mL, R2: 2*15 mL
Origin: Jinan, Shandong
Total Bilirubin (T-Bil) assay kit is applied to measure the concentration of T-Bil in human serum or plasma.


Testing Principle

In the presence of surfactant and accelerating agents, bilirubin can be oxidized by sodium nitrate and converted into biliverdin, which leads to a decrease in absorbance at 450 nm. Total bilirubin concentrations can be calculated from the absorbance change measured at 450 nm.
   
Product Features
 
1. Linear range of T-Bil level: [1, 684] μmol/L
Linear correlation coefficient ∣r∣≥0.990
Linear deviation ≤ ± 10.00%
2. Absorbance of blank ≤ 0.10  
3. Sensitivity
The change in absorbance (ΔmA) ≥ 0.30 while testing the specific T-Bil sample of concentration of 83.1 μmol/L.
4. Accuracy
Relative deviation is within ±10%.
5. Precision
Intra CV% ≤ 5.0%; inter CV% ≤5.0%.

Technical Parameters
 
Temperature 37ºC Optical path length (OPL) 1.0 cm
Main wavelength 450 nm Secondary wavelength 546 nm (Optional)
Method End-point method
(dual reagents) 		Reaction direction Negative
Sample 8 μL R1 240 μL
R2 60 μL Reaction time 300 s
Reference range: 3.42 - 20.50 μmol/L

Packing Specifications

R1: 2×60 ml, R2: 2×15 ml
R1: 4×60 ml, R2: 4×15 ml
R1: 1×40 ml, R2: 1×10 ml (Sample)

Storage and Stability

Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
  Total Bilirubin (T-Bil) assay kit is applied to measure the concentration of T-Bil in human serum or plasma.


Testing Principle

In the presence of surfactant and accelerating agents, bilirubin can be oxidized by sodium nitrate and converted into biliverdin, which leads to a decrease in absorbance at 450 nm. Total bilirubin concentrations can be calculated from the absorbance change measured at 450 nm.
   
Product Features
 
1. Linear range of T-Bil level: [1, 684] μmol/L
Linear correlation coefficient ∣r∣≥0.990
Linear deviation ≤ ± 10.00%
2. Absorbance of blank ≤ 0.10  
3. Sensitivity
The change in absorbance (ΔmA) ≥ 0.30 while testing the specific T-Bil sample of concentration of 83.1 μmol/L.
4. Accuracy
Relative deviation is within ±10%.
5. Precision
Intra CV% ≤ 5.0%; inter CV% ≤5.0%.

Technical Parameters
 
Temperature 37ºC Optical path length (OPL) 1.0 cm
Main wavelength 450 nm Secondary wavelength 546 nm (Optional)
Method End-point method
(dual reagents) 		Reaction direction Negative
Sample 8 μL R1 240 μL
R2 60 μL Reaction time 300 s
Reference range: 3.42 - 20.50 μmol/L

Packing Specifications

R1: 2×60 ml, R2: 2×15 ml
R1: 4×60 ml, R2: 4×15 ml
R1: 1×40 ml, R2: 1×10 ml (Sample)

Storage and Stability

Unopened: Store assay kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).
Opened: Store assay kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.
Do not freeze the assay kit.
 

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