The low-speed refrigerating centrifuge is simple in operation, balanced in operation, stable in performance, safe and reliable. The laboratory can be used in various test tubes, centrifuge tubes, centrifuge bottles, and enzyme-labeled plates. It is multi-purpose and practical. It is widely used in radioimmunity. Production and scientific research units such as biochemistry, blood products, and environmental protection, separating particles with different densities in solution. So how do you operate to improve the separation of low-speed refrigerated centrifuges in specific applications?
1. Precise control of centrifugation time
Centrifugation time is a very important factor affecting the centrifugal effect of a low-speed refrigerated centrifuge. The centrifugation time required for different centrifugation methods will also be different. The following is an example of differential centrifugation and equal density gradient centrifugation and density gradient centrifugation. For differential centrifugation, the centrifugation time refers to the time when a certain particle completely settles to the bottom of the centrifuge tube; for iso-density gradient centrifugation, the centrifugation time refers to the equilibrium time at which the particle completely reaches the iso-density point; the last one Centrifugal time for density gradient centrifugation refers to the time to form well-defined zones. For the latter two centrifugation methods, the required zone formation time or equilibrium time, the influencing factors are complex and can be determined after the test.
2, control the centrifugal speed
The speed of the low-speed refrigerating centrifuge is mainly determined by the rotation speed of the rotor and the radius of rotation of the particles. When describing the centrifugal conditions, it is often expressed by the relative centrifugal force field. In actual work, the data of the centrifugal force field refers to the average value. That is, the centrifugal force field of the particles at the point in the centrifugation solution.
3, pay attention to the pH and temperature of the centrifugal medium
The pH value of the centrifugation medium is generally set within the pH range in which the enzyme is stable. A buffer solution can be used, and the weighing is performed in the test, and the environment of the centrifuge itself is corroded by avoiding an environment of peracid or over-base. When working with a centrifuge, in addition to paying special attention to the choice of centrifugal medium, we must also control the temperature and the pH of the medium solution to prevent agglomeration, denaturation and deactivation of the separated substances. In general, we will control the centrifugation temperature to about 4 ° C. For some enzymes with good thermal stability, centrifugation can also be carried out at room temperature.
This article was edited by the laboratory instrument - Hunan Gilson Technology Development Co., Ltd.
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