Unripe organic fertilizers carry a large number of pathogenic microorganisms and parasitic roundworm eggs. After being applied to farmland, some of them adhere to the crops to cause direct pollution, and some of them enter the soil to cause indirect pollution. In addition, organic fertilizers that have not been cooked before they are applied to the soil must be fermented before they can be absorbed and used by plants. Otherwise, they will not only produce high temperatures and cause burning, but will also release ammonia gas and cause poor plant growth. Therefore, be sure to fully decompose when applying organic fertilizer.
Real Time fluorescence quantitative PCR system is a variation of the standard PCR technique that is commonly used to
quantify DNA or RNA in a sample.
Using sequence-specific primers, the number of copies of a particular DNA or RNA sequence can be determined.
By measuring the amount of amplified product at each stage during the PCR cycle, quantification is possible.
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